Quantification of Y chromosome bearing spermatozoa of cattle using in situ hybridization. |
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Authors: | M Schwerin S Blottner P D Thomsen D Roschlau G Brockmann |
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Affiliation: | Department of Molecular Genetics, Research Center of Animal Production Dummerstorf-Rostock, Federal Republic of Germany. |
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Abstract: | An easy assay for quantification of Y chromosome-bearing sperm (Y-sperm) is needed, especially to monitor sperm separation techniques. In the present study a tritiated bovine male-specific DNA fragment was tested for identification of Y-sperm by in situ hybridization. A protocol for in situ hybridization to bovine sperm was developed and used to study the proportion of Y-sperm of 12 bulls. The usefulness of the method in optimization of sperm separation procedures is illustrated through analysis of fractions of sperm separated by Percoll density gradient centrifugation. |
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Keywords: | Male-specific DNA Spermatozoa Bovine sperm Sexing |
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