The fission yeast Map4 protein is a novel adhesin required for mating |
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Authors: | Sharifmoghadam Mohammad Reza Bustos-Sanmamed Pilar Valdivieso Maria-Henar |
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Affiliation: | Departamento de Microbiología y Genética, Room 231, Instituto de Microbiología Bioquímica, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, CSIC, 37007 Salamanca, Spain. |
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Abstract: | Cell adhesion is required for many cellular processes. In fungi, cell-cell contact during mating, flocculation or virulence is mediated by adhesins, which typically are glycosyl phosphatidyl inositol (GPI)-modified cell wall glycoproteins. Proteins with internal repeats (PIR) are surface proteins involved in the response to stress. In Schizosaccharomyces pombe no adhesins or PIR proteins have been described. Here we study the S. pombe Map4p, which defines a new class of surface protein that is not GPI-modified and has a serine/threonine rich domain and internal repeats that differ from those present in PIR proteins. Map4p is a mating type-specific adhesin required for mating in h(+) cells and enhances cell adhesion when overexpressed. |
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Keywords: | GFP, green fluorescent protein ORF, open reading frame PCR, polymerase chain reaction WT, wild-type GPI, glycosyl phosphatidyl inositol PIR, protein with internal repeats |
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