MicroRNA‐30e‐5p promotes cell growth by targeting PTPN13 and indicates poor survival and recurrence in lung adenocarcinoma |
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| Authors: | Li Zhuang Tao Shou Ke Li Chun‐Lin Gao Lin‐Can Duan Li‐Zhou Fang Qin‐Yong Zhang Zong‐Ning Chen Chao Zhang Shou‐Tao Yang Gao‐Feng Li |
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| Affiliation: | 1. Department of Palliative Medicine, Palliative Medicine Research Center, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China;2. Department of Oncology, The First People's Hospital of Yunnan Province, Kunming, Yunnan Province, China;3. The Second Department of Medicine, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China;4. Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China;5. The Second Department of Respiratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China;6. Department of Pharmacy, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China;7. Department of Cardiology, The People's Hospital of Lijiang City, Lijiang, Yunnan Province, China;8. Cancer Treatment Center, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China;9. Department of Nursing, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China |
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| Abstract: | Aberrant microRNA expression is involved in the regulation of various cellular processes, such as proliferation and metastasis in multiple diseases including cancers. MicroRNA‐30e‐5p (miR‐30e) was previously reported as an oncogenic or tumour suppressing miRNA in some malignancies, but its function in lung adenocarcinoma (LAC) remains largely undefined. In this study, we found that the expression of miR‐30e was increased in LAC tissues and cell lines, associated with tumour size and represented an independent prognostic factor for overall survival and recurrence of LAC patients. Further functional experiments showed that knockdown of miR‐30e suppressed cell growth while its overexpression promoted growth of LAC cells and xenografts in vitro and in vivo. Mechanistically, PTPN13 was identified as the direct target of miR‐30e in LAC, in which PTPN13 expression was down‐regulated in LAC tissues and showed the inverse correlation with miR‐30e expression. Overexpression of PTPN13 inhibited cell growth and rescued the proliferation‐promoting effect of miR‐30e through inhibition of the EGFR signalling. Altogether, our findings suggest that miR‐30e could function as an oncogene in LAC via targeting PTPN13 and act as a potential therapeutic target for treating LAC. |
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| Keywords: | microRNA‐30e‐5p lung adenocarcinoma growth
PTPN13
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