Amphiphilic effects of dibucaine HCl on rotational mobility of n-(9-anthroyloxy)stearic acid in neuronal and model membranes |
| |
Authors: | Lee Young-Ho Park No-Soo Kwon Jong-Deok Park Ju-Seok Shin Gye-Beom Lee Chung-Seob Jung Tae-Sang Choi Nak-Jin Yoon Jin-Hyeok Ok Jin-Seok Yoon Ung-Chan Bae Moon-Kyung Jang Hye-Ock Yun Il |
| |
Institution: | Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Republic of Korea. |
| |
Abstract: | We studied dibucaine's effects on specific locations of n-(9-anthroyloxy)palmitic acid or stearic acid (n-AS) within phospholipids of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex (SPMV) and model membranes. Giant unilamellar vesicles (GUVs) were prepared with total lipids (SPMVTL) and mixture of several phospholipids (SPMVPL) extracted from SPMV. Dibucaine.HCl increased rotational mobility (increased disordering) of hydrocarbon interior, but it decreased mobility (increased ordering) of membrane interface, in both native and model membranes. The degree of rotational mobility in accordance with the carbon atom numbers of phospholipids comprising neuronal and model membranes was in the order at the 16, 12, 9, 6 and 2 position of aliphatic chain present in phospholipids. The sensitivity of increasing or decreasing effect of rotational mobility of hydrocarbon interior or surface region by dibucaine.HCl differed depending on the neuronal and model membranes in the descending order of SPMV, SPMVPL and SPMVTL. |
| |
Keywords: | Fluorescent probe technique Rotational mobility Hydrocarbon interior Membrane interface Dibucaine·HCl |
本文献已被 ScienceDirect PubMed 等数据库收录! |