Glutathione levels during thermal induction of the yeast-to-mycelial transition in Candida albicans |
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Authors: | Donovan Thomas Katherine Klein Elias Manavathu Jonathan R. Dimmock Bulent Mutus |
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Affiliation: | Department of Biological Sciences, University of Windsor, Ontario, Canada. |
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Abstract: | Glutathione (GSH) levels were directly monitored by reverse phase HPLC during the thermal yeast-to-mycelial induction of Candida albicans. The GSH levels decreased approximately 100-fold within 120 min which corresponded to the time of maximal yeast-to-mold conversion. The yeast to mold conversion was inhibited by 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one (CDDP), a thiol-specific alkylator, which prevented the decline in GSH levels. These results are discussed with respect to the potential involvement of intracellular GSH levels in regulation of the yeast-to-mold dimorphism in Candida albicans. |
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Keywords: | Glutathione Yeast to mold transition Candida albicans |
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