The "interceptor" properties of chlorophyllin measured within the three-component system: intercalator-DNA-chlorophyllin |
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| Authors: | Pietrzak Monika Wieczorek Zbigniew Wieczorek Jolanta Darzynkiewicz Zbigniew |
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| Institution: | Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, Poland. |
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| Abstract: | In aqueous solutions, in the presence of double-stranded DNA, chlorophyllin (CHL) forms complexes with each of the three DNA intercalators: acridine orange (AO), quinacrine mustard (QM), and doxorubicin (DOX). The evidence for these interactions was obtained by measurement changes in the absorption and fluorescence spectra of the mixtures containing DNA and intercalators during titration with CHL. A model of simple competition between DNA and CHL for the intercalator was used to define the measured interactions. The concentrations of the complexes estimated based on this model were consistent with the concentrations obtained by actual measurement of the absorption spectra. The present data provide further support for the role of chlorophyllin as an "interceptor" that may neutralize biological activity of aromatic compounds including mutagens and antitumor drugs. |
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| Keywords: | Chlorophyllin Intercalators Acridine orange Quinacrine mustard Doxorubicin Interceptors Competitive interactions Absorption Fluorescence |
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