Isolation and screening of glucose oxidase producing microorganisms from natural sources. |
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Authors: | J Fiedurek Z Ilczuk |
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Institution: | Institute of Microbiology, Maria Curie-Sk?odowska University, Akademicka 19, Lublin, Poland. |
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Abstract: | Among 1486 mould strains isolated from natural sources (screened for extracellular glucose oxidase) only 119 (Aspergillus and Penicillium) showed this enzyme activity. As the best glucose oxidase producer, A. niger 0-1 was isolated from decaying tree. The dynamics of glucose oxidase synthesis in A. niger 0-1 during its culture by submerged method show that the intracellular activity of this enzyme is 10-times higher than its extracellular level. Some properties of the crude glucose oxidase preparation, isolated from the postculture liquids by lyophilization, were examined. |
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