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High cell density cultivation of a recombinant Escherichia coli strain expressing a 6‐O‐sulfotransferase for the production of bioengineered heparin
Authors:J Zhang  M Suflita  CM Fiaschetti  G Li  L Li  F Zhang  JS Dordick  RJ Linhardt
Institution:1. Department of Food Science and Technology, Shanghai Jiao Tong University, Shanghai, China;2. Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA;3. Department of Biology, Rensselaer Polytechnic Institute, Troy, NY, USA;4. Department of Chemistry and Chemical, Biology, Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA;5. Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA
Abstract:
Keywords:biosynthetic enzyme expression  fed‐batch fermentation  heparin  high cell density cultivation  optimization of induction  sulfotransferase
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