Regeneration of NAD(H) covalently bound to formate dehydrogenase with several second enzymes |
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Authors: | Nobuo Kato Tomohide Yamagami Masayuki Shimao Chikahiro Sakazawa |
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Institution: | (1) Department of Environmental Chemistry and Technology, Tottori University, 680 Tottori, Japan |
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Abstract: | Summary
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6-N-(6-Aminohexyl)carbamoylmethyl]-NAD was covalently bound to formate dehydrogenase. The formate dehydrogenase-NAD complex, which contained 0.2 mol of reactive NAD moiety per subunit, functioned as an NAD(H)-regeneration system for a second coupled reaction involving one of the following enzymes; lactate, malate, alanine and leucine dehydrogenases, whose reductive reactions proceeded stoichiometrically in the absence of exogenous NAD. |
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