| 共振瑞利散射测试血清蛋白酸碱度影响的探讨 |
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| 引用本文： | 郭伟平,王高,周汉昌. 共振瑞利散射测试血清蛋白酸碱度影响的探讨[J]. 生物加工过程, 2013, 0(6): 78-81 |
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| 作者姓名： | 郭伟平 王高 周汉昌 |
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| 作者单位： | 中北大学 仪器科学与动态测试教育部重点实验室 电子测试技术国家重点实验室 |
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| 基金项目： | 国家自然科学基金(20576055) |
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| 摘 要： | 提出了一种基于共振瑞利散射（RRS）原理测量人体血清蛋白的新方法。在缓冲溶液的作用下,把配制好的人体血清蛋白稀释液按比例与四羧基酞菁锌混合,经过化学作用后在波长为400 nm左右蓝色波段强光照射下,散射出480 nm左右的共振瑞利散射光强信号。考察在不同pH对共振瑞利散射光强信号与混合物中的血清蛋白反应线性关系的影响。结果表明,pH在6.0～8.0范围内混合溶液共振瑞利散射光强信号与血清蛋白的线性关系良好。
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| 关 键 词： | 共振瑞利散射 血清蛋白质 四羧基酞菁锌 |
Measurement of human serum albumin based on resonance Rayleigh scattering at different pH |
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| GUO Weiping;WANG Gao;ZHOU Hanchang. Measurement of human serum albumin based on resonance Rayleigh scattering at different pH[J]. Chinese Journal of Bioprocess Engineering, 2013, 0(6): 78-81 |
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| Authors: | GUO Weiping WANG Gao ZHOU Hanchang |
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| Affiliation: | GUO Weiping;WANG Gao;ZHOU Hanchang;National Key Laboratory of Electronic Measurement Technology,Key Laboratory of Instrumentation Science & Dynamic Measurement of the Ministry of Education,North University of China; |
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| Abstract: | A method for measuring human hemoglobin by resonance Rayleigh scattering （RRS） was constructed. In buffer solution human hemoglobin and Zn （ II ）-tetracarboxy-phthalocyanine solution was irradiated by 400 nm light. It could scatter resonance Rayleigh scattered light intensity signal of about 480 nm. The linear relation between the resonance Rayleigh scattering light intensity signal and the hemoglobin concentration was established at pH 6.0-8.0. |
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| Keywords: | ： resonance Rayleigh scattering serum protein Zn （ Ⅱ ） -tetracarboxy-phthalocyanine |
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