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抗阿维菌素朱砂叶螨的热激反应及热激蛋白
引用本文:冯宏祖,刘映红,何林,杨大兴,李明,卢文才.抗阿维菌素朱砂叶螨的热激反应及热激蛋白[J].昆虫学报,2008,51(11):1164-1169.
作者姓名:冯宏祖  刘映红  何林  杨大兴  李明  卢文才
作者单位:1. 西南大学植物保护学院,重庆市昆虫学及害虫控制工程重点实验室,重庆,400716;塔里木大学植物科技学院,新疆阿拉尔,843300
2. 西南大学植物保护学院,重庆市昆虫学及害虫控制工程重点实验室,重庆,400716
基金项目:国家自然科学基金项目  
摘    要:选用朱砂叶螨Tetranychus cinnabarinus阿维菌素抗性品系和敏感品系,测定了热预刺激后其在极限高温下的存活率,并应用SDS-PAGE技术研究了热激蛋白(HSPs)的种类及其含量。结果表明:非致死的热预刺激能显著提高朱砂叶螨耐极限温度的能力。两个品系在不同温度热激处理后,其蛋白质种类和含量发生了变化。正常情况下,朱砂叶螨敏感品系与阿维菌素抗性品系相比缺失8条条带;敏感品系热激后,增加了分子量分别为97.2,74.3,62.4,53.0和30.3 kDa的5条条带; 抗性品系热激后没有特异蛋白带的产生,但进一步高温胁迫后有些蛋白表达增强。此结果有助于解释朱砂叶螨抗性品系存在高温适合度优势现象。

关 键 词:朱砂叶螨  阿维菌素  抗性品系  敏感品系  热激反应  热激蛋白  

Heat shock response and HSPs of Tetranychus cinnabarinus(Acari:Tetranychidae) resistant to avermectin
FENG Hong-Zu,LIU Ying-Hong,HE Lin,YANG Da-Xing,LI Ming,LU Wen-Cai.Heat shock response and HSPs of Tetranychus cinnabarinus(Acari:Tetranychidae) resistant to avermectin[J].Acta Entomologica Sinica,2008,51(11):1164-1169.
Authors:FENG Hong-Zu  LIU Ying-Hong  HE Lin  YANG Da-Xing  LI Ming  LU Wen-Cai
Institution:FENG Hong-Zu 1,2,LIU Ying-Hong 1,HE Lin1,YANG Da-Xing1,LI Ming1,LU Wen-Cai1
Abstract:By using methods for insect ecology and SDS-PAGE techniques,the thermotolerance,constituents and contents of heat shock proteins (HSPs) were investigated after the avermectin-resistant and -susceptible strains of Tetranychus cinnabarinus were subjected to heat stress. The results showed that the non-lethal heat-hardening could significantly enhance the ability of tolerance to utmost temperature. After heat shock treatment under different temperatures,the components and contents of HSPs in the two mite strains changed obviously. Compared with the avermectin-resistant strain,eight protein bands were absent in susceptible strain under normal rearing conditions. After heat shock treatment,five additional proteins bands (97.2,74.3,62.4,53.0 and 30.3 kDa) were detected in the susceptible strain. Although no specific protein bands were detected in the resistant strain after heat shock treatment,the expressions of some proteins increased with the continued heat stress. The results may help to explain advantages of the resistant strains of T. cinnabarinu in the fitness to high temperatures.
Keywords:Tetranychus cinnabarinus  avermectin  resistant strain  susceptible strain  heat shock response  heat shock proteins (HSPs)
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