Plasmodium falciparum: IgG subclass antibody response to merozoite surface protein-1 among Amazonian gold miners, in relation to infection status and disease expression |
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Authors: | Scopel Kézia K G Fontes Cor J F Ferreira Marcelo U Braga Erika M |
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Institution: | Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Ant?nio Carlos 6627, 31270-901 Belo Horizonte (MG), Brazil. |
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Abstract: | The merozoite surface protein-1 (MSP-1) of Plasmodium falciparum comprises two major targets of antibody-mediated immunity: the polymorphic block 2 and the 19-kDa C-terminal domain MSP-1(19). Here, we measured antibodies to three block 2 variants and MSP-1(19) among Amazonian gold miners and examined the repertoire of block 2 variants in local parasites. Main findings were as follows: (1) Only seven different block 2 variants were found in 18 DNA sequences analyzed. (2) No major difference was observed in IgG subclass distribution of antibodies from symptomatic P. falciparum-infected patients, asymptomatic parasite carriers, and non-infected subjects. (3) Antibodies to all block 2 antigens, but not to MSP-1(19), were biased towards IgG3 across different strata of cumulative malaria exposure. (4) Similar proportions of symptomatic and asymptomatic subjects failed to recognize the block 2 variant expressed by infecting parasites. These negative results underscore the limits of conventional antibody assays to evaluate clinical immunity to malaria. |
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Keywords: | ANOVA analysis of variance APC antigen presenting cell df degrees of freedom EGF epidermal growth factor ELISA enzyme-linked immunosorbent assay GST glutathione-S-transferase MSP-1 merozoite surface protein-1 MSP-119 the 19-kDa C-terminal domain of merozoite surface proteins-1 PCR polymerase chain reaction RI reactivity index |
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