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Enzymatic peptide synthesis by proline iminopeptidase |
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| Authors: | Takashi Ohshiro Kenichi Mochida Takayuki Uwajima |
| Institution: | (1) Tokyo Research Laboratories, Kyowa Hakko Co., Ltd., 3-6-6, Asahimachi, Machida-shi, 194 Tokyo, Japan;(2) Present address: Department of Biotechnology, Faculty of Engineering, Tottori University, 680 Tottori, Japan;(3) Present address: Makuhari Techno Garden, Novo Nordisk Bioindustry Ltd., CB-6 3 Nakase 1-chome, Chiba-shi, 261-01 Chiba, Japan |
| Abstract: | Summary Proline iminopeptidase was screened for the enzymatic synthesis of peptides containing Pro. The enzyme was purified from the best strainBacillus brevis, and the partially purified enzyme was used for peptide synthesis from Pro-benzylester and Phe. The dipeptide Pro-Phe was obtained in a 40% yield. |
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