Solvent isotope effects on microtubule polymerization and depolymerization |
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Authors: | L L Houston J Odell Y C Lee R H Himes |
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Affiliation: | Department of Biochemistry University of Kansas Lawrence, Kan. 66045, U.S.A. |
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Abstract: | The initial velocity of polymerization of purified beef brain tubulin has been determined at various values of pH or pD in water and in H2O-D2O mixtures. D2O was shown to inhibit both polymerization at 37 °C and depolymerization measured at 5 °C and 37 °C. The microtubules formed in D2O were indistinguishable from those formed in H2O, by electron microscope examination. In 93% D2O the pL2versus rate of polymerization curve was displaced about one unit towards higher pL values. In certain regions of the pL versus rate curve, a stimulation in the rate of polymerization by D2O is observed. The extent of polymerization at the optimum pL value was not affected by D2O. |
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