Observations on the histochemically demonstrable liver glycogen phosphorylase activity and native glycogen under different nutritional conditions |
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Authors: | L. -A. Lindberg |
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Affiliation: | (1) Department of Anatomy and Embryology and the Laboratory of Electron Microscopy, College of Veterinary Medicine, Helsinki;(2) Electron Microscope Laboratory, University of Helsinki, Finland |
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Abstract: | Summary In this study a histochemical demonstration of glycogen phosphorylase activity and native glycogen in the livers from normally fed, overfed and starved rats was performed.It was found that the amount and localization of phosphorylase activity well corresponded to the amount and localization of the native glycogen. A change of the glycogen content in the liver also resulted in a change of the histochemically demonstrable liver glycogen phosphorylase activity.It is concluded that the presence of tissue bound glycogen and undissolved glycogenphosphorylase complexes are necessary for positive histochemical demonstration of liver glycogen phosphorylase activity.This work was supported by a grant from the Finnish Veterinary Medical Foundation. |
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