Imaging interactions of cationic antimicrobial peptides with model lipid monolayers using X-ray spectromicroscopy |
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| Authors: | Bonnie O Leung Adam P Hitchcock Amy Won Anatoli Ianoul Andreas Scholl |
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| Institution: | (1) Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, L8S 4M1, Canada;(2) Department of Chemistry, Carleton University, Ottawa, ON, K1S 5B6, Canada;(3) Advanced Light Source, Berkeley Lab, Berkeley, CA 94720, USA; |
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| Abstract: | The interaction of antimicrobial peptide anoplin with 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol)] lipid monolayers was imaged with atomic force microscopy, scanning transmission X-ray microscopy, and X-ray
photoemission electron microscopy. X-ray absorption spectromicroscopy of the surface revealed the domains of the phase-segregated
surface to be composed of 98(±5)% lipid while the matrix consisted of a ~50:50 lipid-peptide mixture. We show X-ray spectromicroscopy
to be a valuable quantitative tool for label-free imaging of lipid monolayers with antimicrobial peptides at a lateral spatial
resolution below 80 nm. |
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