| 花生幼苗下胚轴质膜Ca2+-ATP酶
及其对低温胁迫的反应 |
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| 作者单位： | ：李美茹 中国科学院华南植物研究所 广州 510650 |
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| 摘 要： | Plasma membrane were obtained from 7-day old
peanu t seedling hypocotyl by 6%～12% Dextran T70 density gradient centrifugation,with
high quality and purity.Plasmalemma ATPase acts normally as hydrolyzing ATP wit hout Mg2+
in reaction system, but the activity was lower than Mg2+- activated ATPase.Ca2+-ATPase
wasn′t inhibited by Na3VO4 and wasn′t activitied by K+,whereas
it is inhibited by Cl-.The optimum pH of Ca2+ -ATPase is different
from that of Mg2+-activated ATPase.Plasma membrane Ca2+-ATPase
activity increased notably under low temperature stress.It is suggest that the possible
role of Ca2+-ATPase is adjusting Ca2+ co ncentration on cytoplasms.
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