Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin |
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| Authors: | S Chattopadhyay AK Srivastava SS Bhojwani VS Bisaria |
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| Institution: | (1) Department of Biochemical Engineering & Biotechnology, Indian Institute of Technology Delhi, Hauz Khas;(2) Department of Botany, Delhi University, Delhi, 110003, India |
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| Abstract: | A suspension culture of Podophyllum hexandrum was established. As the cultures grew, reduction in cell viability, biomass and product yield were associated with browning of culture medium, clumping of cells and drop in medium pH. Supplementation of the medium with both polyvinylpyrrollidone (PVP) and pectinase eliminated these problems. PVP at 10 g l–1 was optimum for both growth of and product formation in P. hexandrum suspension cultures. |
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| Keywords: | podophyllotoxin Podophyllum hexandrum suspension culture |
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