ps-2, the gene responsible for functional sterility in tomato, due to non-dehiscent anthers, is the result of a mutation in a novel polygalacturonase gene |
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Authors: | Benoit Gorguet Danny Schipper André van Lammeren Richard G F Visser Adriaan W van Heusden |
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Institution: | (1) Graduate School of Experimental Plant Sciences, Wageningen UR Plant Breeding, PO Box 386, 6700 AJ Wageningen, The Netherlands;(2) De Ruiter Seeds, Leeuwenhoekweg 52, PO Box 1050, 2660 BB Bergschenhoek, The Netherlands;(3) Graduate School of Experimental Plant Sciences, Wageningen UR Laboratory of Plant Cell Biology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands |
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Abstract: | The recessive mutation ps-2, which appeared spontaneously in tomato, confers functional male sterility due to non-dehiscent anthers. In this study, we
isolated and characterized the PS-2 gene. A single nucleotide mutation in a novel tomato polygalacturonase gene is responsible for the ps-2 phenotype. The mutation in ps-2 is responsible for an alternative splicing during maturation of the pre-mRNA, which leads to an aberrant mRNA. Differentiation
between ps-2 and wild type (PS-2) anthers only appears in the final developmental stage in which the stomium remains closed in the mutant. To our knowledge,
this is the first functional sterility gene isolated in the Solanaceae family. The specific expression of the Arabidopsis homolog of PS-2 in the anther dehiscence zone suggests a conserved mode of action over the plant kingdom, which means that the repression
of PS-2 homologs may be a potential way to introduce functional sterility in other species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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