Symbiotic functioning and bradyrhizobial biodiversity of cowpea (Vigna unguiculataL. Walp.) in Africa |
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| Authors: | Flora Pule-Meulenberg Alphonsus K Belane Tatiana Krasova-Wade Felix D Dakora |
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| Affiliation: | (1) Department of Biotechnology, Tshwane University of Technology, Arcadia Campus, 175 Nelson Mandela Drive, Private Bag X680, 0001 Pretoria, South Africa;(2) Department of Crop Science, Tshwane University of Technology, Arcadia Campus, 175 Nelson Mandela Drive, Private Bag X680, 0001 Pretoria, South Africa;(3) Department of Chemistry, Tshwane University of Technology, Arcadia Campus, 175 Nelson Mandela Drive, Private Bag X680, 0001 Pretoria, South Africa;(4) Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, BP 1386, 18524 Dakar, CP, Senegal |
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| Abstract: | Background Cowpea is the most important food grain legume in Sub-Saharan Africa. However, no study has so far assessed rhizobial biodiversity and/or nodule functioning in relation to strain IGS types at the continent level. In this study, 9 cowpea genotypes were planted in field experiments in Botswana, South Africa and Ghana with the aim of i) trapping indigenous cowpea root-nodule bacteria (cowpea "rhizobia") in the 3 countries for isolation, molecular characterisation using PCR-RFLP analysis, and sequencing of the 16S - 23S rDNA IGS gene, ii) quantifying N-fixed in the cowpea genotypes using the 15N natural abundance technique, and iii) relating the levels of nodule functioning (i.e. N-fixed) to the IGS types found inside nodules. |
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