Micro isoelectric point heterogeneity of a murine monoclonal antibody (L6) originating from cell cultivation conditions |
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| Authors: | B. J. Compton J. S. Gerald D. A. Lowe R. P. Elander |
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| Affiliation: | (1) Bristol-Myers Company Industrial Division, Syracuse, New York;(2) John Hopkins Medical School, Baltimore, Maryland |
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| Abstract: | Summary Isoelectric point (pI) microheterogeneity of an IgG monoclonal antibody (L6) was studied by rapid affinity chromatography, isoelectric focusing on polyacrylamide gels, and rapid ion-exchange chromatography. An abiotic or strictly chemical basis for observed changes is postulated. The described methods are useful for monoclonal antibody process monitoring since they are rapid, automated assay procedures. |
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