| 粉纹夜蛾细胞中Bt Cry1Ac选择抗性的研究及离体品系与毒素结合的比较 |
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| 引用本文： | 刘凯于,蒋才富,洪华珠,彭建新,余泽华.粉纹夜蛾细胞中Bt Cry1Ac选择抗性的研究及离体品系与毒素结合的比较[J].昆虫知识,2003,40(3):247-250. |
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| 作者姓名： | 刘凯于 蒋才富 洪华珠 彭建新 余泽华 |
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| 作者单位： | 华中师范大学昆虫学研究所,武汉,430079 |
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| 基金项目： | 国家自然科学基金资助 (3 90 70 0 14 ) |
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| 摘 要： | 采用Bt Cry1Ac活性毒素对粉纹夜蛾BTI-Tn-5B1细胞进行56代筛选后获得了抗性比为1280倍的抗性细胞。ELISA检测表明抗性细胞总蛋白和膜蛋白结合的Cry1Ac数量都少于敏感细胞。配体结合Western杂交实验显示：抗性细胞和敏感细胞的膜蛋白与总蛋白都有5条电泳迁移率相同的毒素结合多肽带，其分子量分别为207，158．5，118.8，72，38．5 kD；抗性细胞的118．8和72kD的阳性带比敏感细胞的略弱，这可能与抗性的形成相关。
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| 关 键 词： | 粉纹夜蛾 细胞 BtCry1Ac 选择抗性 离体品系 毒素结合 抗性机制 杀虫细菌 苏云金芽孢杆菌 |
| 修稿时间： | 2002年7月19日 |
Comparison of studies on Cry1Ac-binding in resistant and susceptible cells in Trichoplusia ni BTI-Tn-5B1 |
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| LIU Kai\|Yu,JIANG Cai\|Fu,HONG Hua\|Zhu,PENG Jian\|Xin,YU Ze\|Hua.Comparison of studies on Cry1Ac-binding in resistant and susceptible cells in Trichoplusia ni BTI-Tn-5B1[J].Entomological Knowledge,2003,40(3):247-250. |
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| Authors: | LIU Kai\|Yu JIANG Cai\|Fu HONG Hua\|Zhu PENG Jian\|Xin YU Ze\|Hua |
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| Abstract: | The selected\|resistance ratio to Cry1Ac toxin in BTI\|Tn\|5B1 reached 1?280 after 56 generations. ELISA showed that the quantity of Cry1Ac toxin bound by resistant cells was reduced. Western bolt analysis revealed that there were five different molecular weight bands recognized by the Cry1Ac toxin in the membrane proteins of both susceptible and resistant cell's, the molecular weights of which were 207, 158,118\^8,72 and 38\^5 kD respectively. The 118\^8 and 72 kD positive bands from resistant cell samples were weaker than those from susceptible cell samples. The authors suspect that the decrease in the quantity of Cry1Ac toxin bound by 118\^8 and 72 kD peptides on resistant cell membranes is relevant to changes in resistance to this toxin. |
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| Keywords: | Bt Cry1Ac toxin BTI\|Tn\|5B1 cell binding peptide resistance mechanism |
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