A gel-free approach in vascular smooth muscle cell proteome: perspectives for a better insight into activation |
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Authors: | Silvia Rocchiccioli Lorenzo Citti Claudia Boccardi Nadia Ucciferri Lorena Tedeschi Caterina Lande Maria Giovanna Trivella Antonella Cecchettini |
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Affiliation: | (1) Institute of Clinical Physiology, CNR, Via Moruzzi 1, 56124 Pisa, Italy;(2) Department of Human Morphology and Applied Biology, University of Pisa, Via Roma 55, 56126 Pisa, Italy |
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Abstract: | Background The use of chromatography coupled with mass spectrometry (MS) analysis is a powerful approach to identify proteins, owing to its capacity to fractionate molecules according to different chemical features. The first protein expression map of vascular smooth muscle cells (VSMC) was published in 2001 and since then other papers have been produced. The most detailed two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) map was presented by Mayr et al who identified 235 proteins, corresponding to the 154 most abundant unique proteins in mouse aortic VSMC. A chromatographic approach aimed at fractionating the VSMC proteome has never been used before. |
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