Effect of sulfite on antioxidant enzymes and lipid peroxidation in normal and sulfite oxidase-deficient rat erythrocytes |
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Authors: | Oktay Hasan Ozturk Suleyman Oktar Mehmet Aydin Vural Kucukatay |
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Institution: | (1) Faculty of Medicine, Department of Biochemistry, Mustafa Kemal University, 31100 Hatay, Turkey;(2) Faculty of Medicine, Department of Pharmacology, Mustafa Kemal University, 31100 Hatay, Turkey;(3) Faculty of Medicine, Department of Physiology, Mustafa Kemal University, 31100 Hatay, Turkey;(4) Faculty of Medicine, Department of Physiology, Pamukkale University, Kinikli, 20020 Denizli, Turkey |
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Abstract: | Sulfite and related chemical such as sulfite salts and sulfur dioxide has been used as a preservative in food and drugs. This
molecule has also been generated from the catabolism of sulfur-containing amino acids. Sulfite is a very reactive and potentially
toxic molecule and has to be detoxified by the enzyme sulfite oxidase (SOX). The aim of this study was to investigate the
effects of ingested sulfite on erythrocyte antioxidant status by measuring glucose-6-phosphate dehydrogenase (G-6-PD), superoxide
dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and oxidant status by measuring thiobarbituric
acid reactive substances (TBARS) in normal and SOX-deficient rats. Rats were assigned to four groups (n = 10 rats/group) as follows; control (C), sulfite (CS), deficient (D), and deficient + sulfite (DS). SOX deficiency was established
by feeding rats a low molybdenum diet and adding to their drinking water 200 ppm tungsten (W). Sulfite (25 mg/kg) was administered
to the animals via their drinking water. At the end of 6 weeks, Erythrocyte G-6-PD, SOD, and GPx but not CAT activities were
found to be significantly increased with and without sulfite treatment in SOX-deficient groups. Sulfite treatment alone was
also significantly increased erythrocytes’ SOD activity in CS group compared to control. TBARS levels were found to be significantly
increased in CS and DS groups and decreased in D group. When SOX-deficient rats treated with sulfite, TBARS level was still
higher than other groups. In conclusion, these results suggested that erythrocyte antioxidant capacity, a defense mechanism
against the oxidative challenge, increased by endogenous and exogenous sulfite due to its oxidant nature. This increase was
also observed in CS and DS groups but it was insufficient to prevent lipid peroxidation. |
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