Synemin Isoforms in Astroglial and Neuronal Cells from Human Central Nervous System |
| |
Authors: | A. Izmiryan E. Peltekian D. Paulin ZL Li ZG Xue |
| |
Affiliation: | (1) Research Unit 4-Genetic and Physiopathology of Muscular Tissue, University Pierre and Marie Curie, 7 Quai St Bernard, 75005 Paris, France; |
| |
Abstract: | The intermediate filament (IF) synemin gene encodes three IF proteins (H 180, M 150, L 41 kDa) with overlapping distributions. Synemin M was present early with vimentin and nestin. Synemin H was found later in the nervous system and mesodermic derivatives concomitantly with angiogenesis and the migration of neural crest cells. Synemin L appeared later in neurons. A series of in vitro cell cultures were done to identify the linkage between synemin isoforms and specific cell types of the central nervous system (CNS). The neurons and glia from the brains of humans and rats were cultured and double immunostaining done with antibodies against the H/M or L synemin isoforms and neural cell types (βIII-tubulin or NeuN) or astrocyte intermediate filaments (GFAP or vimentin). In neurons of the CNS, synemin H/M were co-expressed with GFAP, vimentin or nestin in glial cells, whereas synemin L was found in neurons. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|