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| Authors: | E. M. Mamontova V. A. Velikov I. V. Volokhina M. I. Chumakov |
| Affiliation: | (1) Plant Sciences Department, The University of Tennessee, 2431 Joe Johnson Drive, Knoxville, TN 37996-4561, USA;(2) USDA/ARS Wheat Genetics, Physiology and Disease Evaluation Unit, Washington State University, 209 Johnson Hall, Pullman, WA 99164-6420, USA |
| Abstract: | A transfer DNA (T-DNA) carrying the marker gene nptII was detected in the genomes of diploid and haploid maize plants obtained after the treatment of pistil filaments with a suspension of Agrobacterium during artificial pollination. PCR analysis of total DNA isolated from 155 canamycin-resistant diploid F1 seedlings revealed T-DNA insertions in the genomes of 111 plants (32.7% of the total number of analyzed seeds). The example of matroclinal haploids was used to demonstrate that T-DNA may be transported to the egg cell by the growing pollen tube (PT). Twelve out of 16 analyzed haploid plants contained the T-DNA insertion. The possible mechanism of the transfer of the Agrobacterium T-DNA to the maize genome during pollination is discussed. |
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