Large impact of the apoplast on somatic embryogenesis in Cyclamen persicum offers possibilities for improved developmental control in vitro |
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Authors: | Claudia Hoenemann Sandra Richardt Katja Krüger Andreas D Zimmer Annette Hohe Stefan A Rensing |
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Affiliation: | (1) Department Plant Propagation, Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), Kuehnhaeuser Strasse 101, 99189 Erfurt, Germany;(2) University of Freiburg, Faculty of Biology, Hauptstrasse 1, 79104 Freiburg, Germany;(3) QIAGEN GmbH, Qiagenstrasse 1, D-40724 Hilden, Germany;(4) University of Freiburg, Faculty of Biology, Plant Biotechnology, Schaenzlestrasse 1, 79104 Freiburg, Germany |
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Abstract: | Background Clonal propagation is highly desired especially for valuable horticultural crops. The method with the potentially highest multiplication rate is regeneration via somatic embryogenesis. However, this mode of propagation is often hampered by the occurrence of developmental aberrations and non-embryogenic callus. Therefore, the developmental process of somatic embryogenesis was analysed in the ornamental crop Cyclamen persicum by expression profiling, comparing different developmental stages of embryogenic cell cultures, zygotic vs. somatic embryos and embryogenic vs. non-embryogenic cell cultures. |
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