Unfixed Material Embedded in A Methacrylate Resin (Technovit 7100) For Immunofluorescent Staining |
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| Authors: | Wolfgang Popp Hartmut Zwick |
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| Institution: |
a Pulmonary Division-KH Lainz, University of Vienna, Institute of Histology and Embryology, Vienna, Austria |
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| Abstract: | Wheat grains were embedded with or without fixation in Technovit 7100 or in paraffin. This enabled us to produce 4 μm sections for imnwnofluorescent staining to see whether serum of patients with baker's asthma contained IgG antibodies against wheat grains. Embedding without fixation in Technovit 7100 appeared to be suitable for unmunofluorescent staining and gave superior results to protocols requiring fixation. |
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