Identification and isolation of the yeast cytochrome c gene. |
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Authors: | D L Montgomery B D Hall S Gillam M Smith |
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Affiliation: | Genetics Department SK-50 University of Washington Seattle, Washington 98195 USA;Department of Biochemistry Faculty of Medicine University of British Columbia Vancouver, British Columbia VGT 1W5 Canada |
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Abstract: | The iso-1-cytochrome c gene of yeast has been identified and cloned using a synthetic oligodeoxynucleotide as a hybridization probe. The oligomer d[pT-T-A-G-C-A-G-A-A--C-C-G-G] is complementary to a region near the N terminal coding region of the yeast cyc 1 gene. Of several yeast Eco RI fragments which hybridize to this probe, one is changed in size by a G leads to T mutation which eliminates an Eco RI site within the cyc 1 gene. Both the wild-type and the RI- mutant forms were cloned in lambda gt vectors. Maxam-Gilbert sequencing for 91 nucleotides into the coding region for iso-1-cytochrome c yielded a DNA sequence in perfect correspondence with the known protein sequence. |
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