2007年北京市急性出血性结膜炎的病原与分子进化分析

2007年北京市发生了急性出血性结膜炎(Acute Hemorrhagic Conjunctivitis,AHC)疫情。为鉴定引起这次疫情的致病病原体,采集北京市门诊就诊的AHC患者眼结膜拭子标本共57份,使用PCR或RT-PCR法分别检测临床标本中腺病毒、人肠道病毒70型(Human Enterovirus 70,HEV70)和柯萨奇病毒A组24型变种(CoxsackievirusA24 variant,CVA24v)的基因,其中有38份检测结果为CVA24v阳性,阳性率为66.7%,而腺病毒和HEV70检测结果均为阴性,说明引起本次AHC流行的病原体是CVA24v。使用HEp-2细胞共分离到9株病毒,通过分子定型均鉴定为CVA24v,对9株CVA24v进行全长VP1区扩增和核苷酸序列测定和分析后,发现除0744/BJ/CHN/2007株之外,其它8株在全长VP1区核苷酸水平和氨基酸水平上差异都很小,同源性分别大于99.6%和100.0%,而0744/BJ/CHN/2007株与其它8株CVA24v的核苷酸和氨基酸同源性分别为96.8%~97.2%和99.7%。进化树图显示,2007年北京CVA24v分离株与...

Outbreak of Acute Hemorrhagic Conjunctivitis in Beijing City in 2007 Caused by Coxsackievirus A24 Variant: Molecular Identification and Phylogenetic Analysis

In 2007,an outbreak of acute hemorrhagic conjunctivitis(AHC) occurred in Beijing.In order to identify the etiology of this outbreak,57 eye conjunctival swabs were collected from 57 outpatient patients,and detected for adenovirus,human enterovirus 70(HEV70) and Coxsackievirus A24 variant(CVA24v) genes by using RT-PCR or PCR methods.The results showed that 38 were positive for CVA24v,the positive rate was 66.7%,but none was positive for HEV70 and adenovirus,showing that this outbreak was caused by CVA24v.9 vi...