|
|||||
|
|
Rapid identification of Streptococcus pneumoniae by PCR amplification of ribosomal DNA spacer region |
|
| Authors: | Katsutoshi Saruta Teiichi Matsunaga Sadayori Hoshina Midori Kono Shintaro Kitahara Sonomi Kanemoto Osamu Sakai Katsuhiko Machida |
| Institution: | Department of Laboratory Medicine, Jikei University School of Medicine, 3-25-8 Nishishinbashi, Minato-ku, Tokyo 105, Japan; Department of Internal Medicine (II), Jikei University School of Medicine, 3-25-8 Nishishinbashi, Minato-ku, Tokyo, 105, Japan |
| Abstract: | Abstract Streptococcus pneumoniae is one of the important human pathogens in clinical microbiology. A polymerase chain reaction assay was designed to detect and identify S. pneumoniae through amplification of the ribosomal DNA spacer regions between the pneumococcal 16S-23S ribosomal RNA genes. Thirty-two Streptococcus and non- Streptococcus strains were tested to verify the specificity of the assay, and only S. pneumoniae strains gave a positive reaction. This method is a powerful technique for the rapid identification of S. pneumoniae . |
| Keywords: | Polymerase chain reaction Ribosomal DNA spacer region Streptococcus pneumoniae |