The effect of sodium channel activators on muscarinic receptors of neuroblastoma cells |
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Authors: | G Milligan P G Strange |
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Affiliation: | Department of Biochemistry, The Medical School, Queen''s Medical Centre, Nottingham, NG7 2UH, England |
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Abstract: | Incubation of neuroblastoma NIE 115 cells with veratrine leads to an apparent reduction in the number of muscarinic acetylcholine receptors assayed by [3H]scopolamine methyl chloride binding. No true down-regulation of the receptors occurs but a component of veratrine with muscarinic receptor affinity, which is not veratridine, enters the intracellular water space during the incubation period and competes with [3H]scopolamine methyl chloride for the muscarinic binding sites in subsequent ligand binding assays unless it is carefully washed away. Treatment of cells with the agonist carbamoylcholine does, however, lead to a true downregulation of muscarinic receptors. |
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Keywords: | Muscarinic receptor Neuroblastoma cell Veratridine Veratrine |
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