硝酸银对雌性黄瓜植株三种氧化酶同工酶的影响

雌性黄瓜植株经硝酸银处理后其茎尖和真叶过氧化物酶活性极显著地增加，茎尖２４小时、真叶３６小时酶活性达到最大值，分别增加１７８．２％和２８４．６％，随后酶活性逐渐下降，但酶活性仍然较对照植株高。多酚氧化酶和超氧化物歧化酶的同工酶活性也增加。同时硝酸银能诱发黄瓜植株过氧化物酶、多酚氧化酶和超氧化物歧化酶产生新的同工酶。用等电聚焦更能有效地观察新产生的同工酶。

The Effect of Silver Nitrate on Three Oxidase Isozymes of Gynoecious Cucumber Plant (Cucumis sativus L.)

The activity of peroxidase in the shoot tips and the leaves of gynoecious cucumb er plants increased significantly after treatment of silver nitrate. The maximu m increment was 178.2% in the shoot tips after 24 hours and 284.6% in leaves aft e r 36 hours. Meanwhile, new isozyme bands in peroxidase, polyphenol oxidase and s uperoxide dismutase were developed by silver nitrate treatment. It is considere d that the response of cucumber plants to silver ion injury resulted in the risi ng of activity and the production of new oxidase isozymes.