Linear alkanesulfonates as carbon and energy sources for gram-positive and gram-negative bacteria |
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Authors: | Wolfram Reichenbecher J Colin Murrell |
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Institution: | (1) Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK e-mail: cm@dna.bio.warwick.ac.uk, Tel.: +44-1203-523553, Fax: +44-1203-523701, GB |
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Abstract: | Several bacteria from soil and rainwater samples were enriched and isolated with propanesulfonate or butanesulfonate as sole
carbon and energy source. Most of the strains isolated utilized nonsubstituted alkanesulfonates with a chain length of C3–C6
and the substituted sulfonates taurine and isethionate as carbon and energy source. A gram-positive isolate, P40, and a gram-negative
isolate, P53, were characterized in more detail. Phylogenetic analysis grouped strain P40 within group IV of the genus Rhodococcus and showed a close relationship with Rhodococcus opacus. After phylogenetic and physiological analyses, strain P53 was identified as Comamonas acidovorans. Both bacteria also utilized a wide range of sulfonates as sulfur source. Strain P40, but not strain P53, released sulfite
into the medium during dissimilation of sulfonated compounds. Cell-free extracts of strain P53 exhibited high sulfite oxidase
activity 2.34 U (mg protein)–1] when assayed with ferricyanide, but not with cytochrome c. Experiments with whole-cell suspensions of both strains showed that the ability to dissimilate 1-propanesulfonate was specifically
induced during growth on this substrate and was not present in cells grown on propanol, isethionate or taurine. Whole-cell
suspensions of both strains accumulated acetone when oxidizing the non-growth substrate 2-propanesulfonate. Strain P40 cells
also accumulated sulfite under these conditions. Stoichiometric measurements with 2-propanesulfonate as substrate in oxygen
electrode experiments indicate that the nonsubstituted alkanesulfonates were degraded by a monooxygenase. When strain P53
grew with nonsubstituted alkanesulfonates as carbon and energy source, cells expressed high amounts of yellow pigments, supporting
the proposition that an oxygenase containing iron sulfur centres or flavins was involved in their degradation.
Received: 21 December 1998 / Accepted: 18 March 1999 |
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Keywords: | Comamonas acidovorans Rhodococcus Alkanesulfonates Monooxygenases Sulfonates |
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