Effect of age and day time on the adenosine modulation of basal and insulin-stimulated glucose transport in rat adipocytes |
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Authors: | P Bush J E Souness V Chagoya de Sánchez |
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Affiliation: | Departamento de Bioenergética, Universidad Nacional Autónoma de México, D.F. México. |
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Abstract: | 1. The relationship between the activity of adenosine metabolizing enzymes 5'nucleotidase (5'N), adenosine kinase (A.K.) and adenosine deaminase (A.D.) with basal and insulin-stimulated glucose transport in isolated fat cells from young and old animals was studied at 08:00 and 16:00 hr. 2. In cells from young animals a larger insulin-stimulation of glucose transport was observed at 16:00 hr than at 08:00 hr. Also at 16:00 hr small changes in 5'N, A.K. and A.D. activities suggest a decrease in adenosine formation. 3. In the cells from old animals no effect of insulin was observed at any time, while a 3-5-fold increase in 5'N indicated a predominance of adenosine formation at both times studied. 4. An inverse relationship was observed in the changes of adenosine metabolism and insulin action. |
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