X-linked gene expression in metatherian fibroblasts: Evidence from theGpd andPgk-A loci of the Virginia opossum and the red-necked wallaby |
| |
Authors: | Paul B. Samollow Peter G. Johnston Allen L. Ford John L. VandeBerg |
| |
Affiliation: | (1) Department of Biology, Williams Hall No. 31, Lehigh University, 18015-3189 Bethlehem, Pennsylvania;(2) Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284 San Antonio, Texas;(3) School of Biological Sciences, Macquarie University, 2109 Sydney, New South Wales, Australia |
| |
Abstract: | Fibroblasts cultured from ear pinna biopsies of Virginia opossums (Didelphis virginiana) and red-necked wallabies (Macropus rufogriseus) were examined electrophoretically to determine the relative expression levels of the maternally and paternally derived alleles at X-linked, enzyme-coding loci. Only the maternally derived allele was expressed at thePgk-A locus in fibroblasts of heterozygousD. virginiana (M. rufogriseus not examined), but fibroblasts of both species exhibited evidence of paternal allele expression a t theGpd locus. Furthermore, the heterozygous G6PD phenotypes in both species were skewed in favor of the maternal gene product, as expected if the paternal allele is only partially (incompletely) expressed. ForM. rufogriseus this result is contrary to a previous finding which suggested equal expression of bothGpd alleles in cultured fibroblasts of this species. The present results suggest that X-linked genes in metatherian fibroblasts are subject to the same kind of determinate, paternal allele inactivation, incomplete at some loci, described previously for X-linked genes in adult tissues and that the pattern of paternal X-linked gene expression in these cells is independent of the patterns in the tissues from which the fibroblasts are derived.The work was supported in part by grants from the National Institutes of Health (Biomedical Research Support Grant RR-05519) and the National Science Foundation (DCB 8516949). |
| |
Keywords: | X-chromosome inactivation marsupials cultured fibroblasts glucose-6-phosphate dehydrogenase phosphoglycerate kinase-A |
本文献已被 SpringerLink 等数据库收录! |
|