Species and sex identification of western lowland gorillas (Gorilla gorilla gorilla), eastern lowland gorillas (Gorilla beringei graueri) and humans |
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| Authors: | Miki Matsubara A Kanyunyi Basabose Ilambo Omari Kiswele Kaleme Byamana Kizungu Kiyego Sikubwabo Muzusangabo Kahindo Juichi Yamagiwa Osamu Takenaka |
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| Institution: | (1) Laboratory of Human Evolution Studies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan;(2) Centre de Recherches en Science Naturelles at Lwiro, D. R. C. C/O Petit Seminaire de Mugeri, B.P.02, Cyangugu, Rwanda;(3) Parc National de Kahuzi-Biega, Bukavu, D.R. Congo;(4) Makerere University, P.O.Box 7062, Kampala, Uganda;(5) Molecular Biology Section, Primate Research Institute, Kyoto University, 41 Kanrin, Inuyama, Aichi 484-8506, Japan |
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| Abstract: | Methods for the identification of the sex and species of individuals from samples non-invasively taken from humans and gorillas were established. Amplification of a segment of amelogenin (AMG), which is an X–Y homologous gene, using two pairs of primers from human AMG, revealed both X- and Y-specific bands. The possibility of sex identification was examined by typing the AMG gene using hair and fecal samples from captive western lowland gorillas (Gorilla gorilla gorilla) in Japan and hair samples from wild eastern lowland gorillas (Gorilla beringei graueri) in the Kahuzi-Biega National Park, Democratic Republic of Congo, which were sexed by direct observation. Species-specific bands of AMG in gorillas and humans were identified by restriction fragment length polymorphisms analysis. These tests could be used for sexing unidentified individuals of wild western and eastern lowland gorillas, and screening contamination of human DNA from non-invasively acquired samples. |
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| Keywords: | Amelogenin Gorillas PCR– RFLP Sex identification Species identification |
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