In vitro antimicrobial activity of light-activated phthalocyanines |
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Authors: | Lenka Ryskova Vladimir Buchta Marie Karaskova Jan Rakusan Jiri Cerny Radovan Slezak |
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Institution: | 1. Department of Clinical Microbiology, Faculty of Medicine and University Hospital, 500 05, Hradec Kralove, Czech Republic 2. Research Institute for Organic Syntheses, 533 54, Rybitv??, Czech Republic 3. Department of Dentistry, Faculty of Medicine and University Hospital, 500 05, Hradec Kralove, Czech Republic
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Abstract: | Background Photodynamic antimicrobial therapy (PACT) is proposed as a topical, non-invasive approach suitable for treatment of locally occurring infection. Research of photosensitizers, (PS) as well as their development, is aimed at finding effective antimicrobial substances which would have a broad-spectrum potency. The aim of this paper is to evaluate the antimicrobial effect of phthalocyanine (Pc) derivatives. Methods Fifteen different Pc compounds were investigated. Their photokilling activity was tested on Staphylococcus aureus, Escherichia coli and Candida albicans. After treating of microbial cells with Pc at the concentrations: 1 mg/l, 2 mg/l, 4 mg/l, 8 mg/l for 30 minutes, the cultures were irradiated with low-power laser light at a wavelength of 670 nm (20 J/cm2, 40 J/cm2). The effectiveness of photoinactivation was evaluated based on the decrease in number (log10) of viable bacteria. Results Eight Pc compounds tested showed antibacterial effects against S. aureus, but only four were effective against E. coli and two against C. albicans. The most effective photosensitizers were amphiphilic sulphonated zinc Pc compounds (3-diethylammonium)-propylsulphonamide citrate (Pc3) and cationic tetramethylenepyridinium chloride of hydroxyaluminum Pc (Pc7)]. Conclusions The most efficient phthalocyanines (Pc3, Pc7) cause a significant decrease in viable counts of all tested microbes. |
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