Tracing pollen nuclei in the ovary and ovule of Gasteria verrucosa (Mill.) H. Duval after pollination with DAPI-stained pollen |
| |
Authors: | M. T. M. Willemse C. J. Keijzer |
| |
Affiliation: | (1) Department of Plant Cytology and Morphology, Agricultural University, Arboretumlaan 4, NL-6703 BD Wageningen, The Netherlands |
| |
Abstract: | Summary The addition of DAPI particles to the stigma exudate of Gasteria results in the labelling of the pollen nuclei. By means of epifluorescence microscopy and clearing of the ovule, the labelled nuclei of the sperm cells and, subsequently, the zygotic nucleus can be observed. The method was used in a cross-pollination with low seed setting to examine different types of penetration and transport of the pollen tube nucleus and sperm cell nuclei. More than one pollen tube can penetrate, but generally only one set of sperm cell nuclei is accepted. |
| |
Keywords: | DAPI labelling Pollen tube penetration Sperm cell nuclei Egg apparatus Gasteria |
本文献已被 SpringerLink 等数据库收录! |