The nuclear ceramide/diacylglycerol balance depends on the physiological state of thyroid cells and changes during UV-C radiation-induced apoptosis |
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Authors: | Albi E Cataldi S Rossi G Viola Magni M Toller M Casani S Perrella G |
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Institution: | a Department of Clinical and Experimental Medicine, Physiopathology Section, Policlinico Monteluce, Via Brunamonti, 06100 Perugia, Italy b Department of Experimental and Clinical Pathology and Medicine, University of Udine, Italy |
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Abstract: | Intranuclear lipid metabolism modifications in relation to cell proliferation and/or apoptosis were demonstrated in hepatocytes. The aim of this study was to establish whether nuclear lipid metabolites influence cell function in different experimental models using a rat thyroid cell line (FRTL-5) treated with UV-C radiation. After UV-C irradiation cells proliferate and undergo apoptosis in the presence of thyrotropin, are quiescent and resistant to radiation-induced apoptosis in its absence and finally are proapoptotic for nutrition withdrawal. In nuclei purified from proliferating cells, irradiation stimulates neutral-sphingomyelinase activity and inhibits sphingomyelin-synthase, phosphatidylcholine-specific phospholipase C and phosphatidylinositol-specific phospholipase C activity with a consequent increase in the ceramide/diacylglycerol ratio. This effect is marked in proapoptotic cell nuclei and low in quiescent cell nuclei. In conclusion, UV-C radiation induces apoptosis, modifying nuclear lipid metabolism in relation to the physiological state of cells. |
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Keywords: | Apoptosis Ceramide Neutral-sphingomyelinase Nucleus Reverse-sphingomyelin-synthase Sphingomyelin-synthase Thyroid cell Ultraviolet rays |
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