Functional characterization of a wheat plasma membrane Na+/H+ antiporter in yeast |
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Authors: | Xu Haixia Jiang Xingyu Zhan Kehui Cheng Xiyong Chen Xinjian Pardo Jose M Cui Dangqun |
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Institution: | a College of Agronomy, Henan Agricultural University, Wenhua Road 95, Zhengzhou, Henan 450002, China b Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas, Avda Reina Mercedes 10, Sevilla 41012, Spain |
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Abstract: | The functional analysis of the sodium exchanger SOS1 from wheat, TaSOS1, was undertaken using Saccharomyces cerevisiae as a heterologous expression system. The TaSOS1 protein, with significant sequence homology to SOS1 sodium exchangers from Arabidopsis and rice, is abundant in roots and leaves, and is induced by salt treatment. TaSOS1 suppressed the salt sensitivity of a yeast strain lacking the major Na+ efflux systems by decreasing the cellular Na+ content while increasing K+ content. Na+/H+ exchange activity of purified plasma membrane from yeast cells expressing TaSOS1 was higher than controls transformed with empty vector. These results demonstrate that TaSOS1 contributes to plasma membrane Na+/H+ exchange. |
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Keywords: | Na+/H+ antiporter Plasma membrane Saccharomyces cerevisiae Salt stress Triticum aestivum |
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