| 细菌脲酶蛋白复合物及其活化机制 |
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| 引用本文： | 李晓姣,赵圣国,郑楠,程建波,王加启.细菌脲酶蛋白复合物及其活化机制[J].生物工程学报,2019,35(2):204-215. |
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| 作者姓名： | 李晓姣 赵圣国 郑楠 程建波 王加启 |
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| 作者单位： | 1 中国农业科学院北京畜牧兽医研究所 动物营养学国家重点实验室，北京 100193;2 中国农业科学院北京畜牧兽医研究所 农业农村部奶产品质量安全风险评估实验室，北京 100193;3 安徽农业大学 动物科技学院，安徽 合肥 230036,1 中国农业科学院北京畜牧兽医研究所 动物营养学国家重点实验室，北京 100193;2 中国农业科学院北京畜牧兽医研究所 农业农村部奶产品质量安全风险评估实验室，北京 100193,1 中国农业科学院北京畜牧兽医研究所 动物营养学国家重点实验室，北京 100193;2 中国农业科学院北京畜牧兽医研究所 农业农村部奶产品质量安全风险评估实验室，北京 100193,3 安徽农业大学 动物科技学院，安徽 合肥 230036,1 中国农业科学院北京畜牧兽医研究所 动物营养学国家重点实验室，北京 100193;2 中国农业科学院北京畜牧兽医研究所 农业农村部奶产品质量安全风险评估实验室，北京 100193 |
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| 基金项目： | 国家自然科学基金 (Nos. 31501981, 31430081)，中国农业科学院科技创新工程 (No. ASTIP-IAS12)，现代农业产业技术体系专项资金(No. CARS-37) 资助。 |
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| 摘 要： | 脲酶能够催化尿素分解生成氨,在农业和医学领域中具有重要的意义。细菌脲酶蛋白包括结构蛋白(UreA、UreB和UreC)和辅助蛋白(UreD/UreH、UreE、UreF和UreG),它们在脲酶活化过程中各自具有独特的作用,结构蛋白形成脲酶活性中心,而辅助蛋白主要负责镍离子的传递。文中综述了细菌脲酶蛋白复合物的结构和功能,以及各蛋白之间如何相互作用完成其活化过程,以期为脲酶活性调控研究及脲酶抑制剂开发等提供理论指导。
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| 关 键 词： | 细菌脲酶，结构蛋白，辅助蛋白，活化机制 |
| 收稿时间： | 2018/6/10 0:00:00 |
Progress in bacterial urease complexes and their activation mechanisms |
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| Xiaojiao Li,Shengguo Zhao,Nan Zheng,Jianbo Cheng and Jiaqi Wang.Progress in bacterial urease complexes and their activation mechanisms[J].Chinese Journal of Biotechnology,2019,35(2):204-215. |
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| Authors: | Xiaojiao Li Shengguo Zhao Nan Zheng Jianbo Cheng and Jiaqi Wang |
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| Institution: | 1 State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2 Laboratory of Quality & Safety Risk Assessment for Dairy Products of China Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;3 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China,1 State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2 Laboratory of Quality & Safety Risk Assessment for Dairy Products of China Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China,1 State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2 Laboratory of Quality & Safety Risk Assessment for Dairy Products of China Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China,3 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China and 1 State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2 Laboratory of Quality & Safety Risk Assessment for Dairy Products of China Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | Urease decomposes urea to ammonia, and has application potential in agriculture and medical treatment. Urease proteins include structural proteins (UreA, UreB and UreC) and accessory proteins (UreD/UreH, UreE, UreF and UreG), each of them has its own unique role in urease maturation. The structural proteins form the active center of urease, and the accessory proteins are responsible for the delivery of nickel. We review here the structure and function of bacterial urease complexes, and how each protein interacts to complete the activation process. We hope to provide theoretical basis for the regulation of urease activity and the development of urease inhibitors. |
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| Keywords: | bacterial urease structural protein accessory protein activation mechanism |
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