| 类鼻疽伯克霍尔德菌bopA基因敲除株的构建及生物学特征 |
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| 引用本文： | 卢晓雪,胡治强,梅国徽,张雨,胡语涵,岳娟娟,向阳,毛旭虎. 类鼻疽伯克霍尔德菌bopA基因敲除株的构建及生物学特征[J]. 微生物学通报, 2019, 46(2): 233-242 |
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| 作者姓名： | 卢晓雪 胡治强 梅国徽 张雨 胡语涵 岳娟娟 向阳 毛旭虎 |
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| 作者单位： | 1 陆军军医大学(第三军医大学)药学与检验医学系临床微生物与免疫学教研室 重庆 400038,1 陆军军医大学(第三军医大学)药学与检验医学系临床微生物与免疫学教研室 重庆 400038,2 陆军勤务学校门诊部 重庆 401331,1 陆军军医大学(第三军医大学)药学与检验医学系临床微生物与免疫学教研室 重庆 400038,1 陆军军医大学(第三军医大学)药学与检验医学系临床微生物与免疫学教研室 重庆 400038,1 陆军军医大学(第三军医大学)药学与检验医学系临床微生物与免疫学教研室 重庆 400038,1 陆军军医大学(第三军医大学)药学与检验医学系临床微生物与免疫学教研室 重庆 400038,1 陆军军医大学(第三军医大学)药学与检验医学系临床微生物与免疫学教研室 重庆 400038 |
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| 基金项目： | 国家自然科学基金(81471914，81601832) |
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| 摘 要： | 【【背景】类鼻疽杆菌是一种能够引起人类疾病甚至死亡的胞内寄生菌,Ⅲ型分泌系统在该菌入侵上皮细胞、逃避宿主免疫以及毒力因子的分泌过程中发挥重要作用,其中bopA基因为TTSS-3基因编码的重要效应蛋白,在类鼻疽杆菌的免疫逃逸中发挥重要作用。【目的】构建类鼻疽杆菌bopA基因敲除菌株,并对其生物学特征进行初步研究。【方法】构建pK18mobSacB-ΔbopA自杀质粒,通过大肠杆菌S17-1λpair以接合的方式转入类鼻疽杆菌,利用同源重组敲除了bopA基因,并用蔗糖平板筛选出菌株,最后在细胞和动物水平检测敲除菌株的表型变化。【结果】构建了bopA敲除的类鼻疽菌株,并通过细胞和动物实验证实敲除bopA基因后,细菌的细胞侵袭和胞内存活以及体内定殖能力都显著降低。【结论】利用同源重组成功构建类鼻疽bopA基因敲除株,为深入研究该基因的作用靶点奠定了实验基础。
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| 关 键 词： | 类鼻疽伯克霍尔德菌，bopA基因，基因敲除，生物学特征 |
Construction and characterization of Burkholderia pseudomallei bopA gene knockout mutant |
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| LU Xiao-Xue,HU Zhi-Qiang,MEI Guo-Hui,ZHANG Yu,HU Yu-Han,YUE Juan-Juan,XIANG Yang and MAO Xu-Hu. Construction and characterization of Burkholderia pseudomallei bopA gene knockout mutant[J]. Microbiology China, 2019, 46(2): 233-242 |
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| Authors: | LU Xiao-Xue HU Zhi-Qiang MEI Guo-Hui ZHANG Yu HU Yu-Han YUE Juan-Juan XIANG Yang MAO Xu-Hu |
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| Affiliation: | 1 Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Army Medical University, (Third Military Medical University), Chongqing 400038, China,1 Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Army Medical University, (Third Military Medical University), Chongqing 400038, China,2 Affiliated Hospital, Army Logistic University of PLA, Chongqing 401331, China,1 Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Army Medical University, (Third Military Medical University), Chongqing 400038, China,1 Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Army Medical University, (Third Military Medical University), Chongqing 400038, China,1 Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Army Medical University, (Third Military Medical University), Chongqing 400038, China,1 Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Army Medical University, (Third Military Medical University), Chongqing 400038, China and 1 Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Army Medical University, (Third Military Medical University), Chongqing 400038, China |
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| Abstract: | |
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| Keywords: | Burkholderia pseudomallei bopA gene Gene knockout Biological characteristics |
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