东北湿地沉积物中T4型噬菌体g23基因的多样性

【目的】揭示我国东北典型湿地沉积物中T4型噬菌体g23基因的多样性,明确湿地环境T4型噬菌体群落分布特征,为噬菌体生态学研究提供数据支撑。【方法】采用简并性引物MZIA1bis和MZIA6对采自东北6个地点不同类型湿地沉积物土壤DNA进行PCR扩增,采用克隆测序方法,解析沉积物中T4型噬菌体g23基因组成,通过UniFrac分析T4型噬菌体群落结构在湿地沉积物中与其他环境中的差异。【结果】在东北湿地沉积物中共得到262条不同的g23基因序列,构建的系统进化树分析表明,我国东北湿地沉积物T4型噬菌体g23基因分布与海洋、湖泊及稻田生态系统中g23基因亲缘关系较近,而与东北旱地黑土g23基因分布较远;以g23基因群集表征的T4型噬菌体群落在不同地点湿地中分异明显。【结论】东北湿地生态系统T4型噬菌体群落结构复杂多样,存在着一些未知的噬菌体类群。

T4-type bacteriophage genetic diversity in wetland sediments in northeast China

Objective] To provide scientific data for studying the ecology of bacteriophage in natural environments, the genetic diversity and distribution patterns of g23 of T4-type bacteriophage in wetland sediments were revealed.Methods] Degenerate primers MZIA1bis and MZIA6 were used to PCR amplification of g23 gene of T4-type bacteriophages, and the PCR products were cloned and sequenced to reveal the diversity of g23 sequences in 6 different wetland sediment samples obtained from northeast China. In addition, the Unifrac analysis was used to compare the T4-type bacteriophage community structures in wetland sediments with those in other environments.Results] In total, 262 different g23 clones of T4-type bacteriophage were obtained. Neighbor-joining phylogenetic tree showed that the distribution of g23 genes in wetland sediments was similar to those in aquatic ecosystems such as oceans and lakes and paddy ecosystems, but distinctly different from those in upland black soils. In addition, the T4-type bacteriophage community as evaluated by g23 gene assembles was remarkably different among different wetlands.Conclusion] The composition of T4-type bacteriophage community was complex and diverse in wetland ecosystem, and the novel g23 groups of T4-type bacteriophage existed in wetland sediments in northeast China.