Use of in vitro cultures of Nicotiana benthamiana for the purification of grapevine virus A |
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Authors: | Paul L. Monette Delano James |
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Affiliation: | (1) Agriculture Canada, Plant Quarantine Station, 8801 East Saanich Road, V8L 1H3 Sidney, British Columbia, Canada |
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Abstract: | Seedlings of Nicotiana benthamiana were inoculated with grapevine virus A (GVA). Three weeks later, upon systemic symptom expression, cultures were established in vitro using single nodes from these plants. GVA was purified from these cultures and from leaves of GVA-infected N. benthamiana plants maintained in the greenhouse. More virus was obtained from the proliferating in vitro node cultures than from the leaves. The use of in vitro cultures for virus purification represents a valuable practical application of plant tissue culture techniques. |
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Keywords: | grapevine virus A in vitro node culture Nicotiana benthamiana virus purification |
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