Calcium-modulated desmosome formation and sodium-regulated keratinization in frog skin cultures |
| |
| Authors: | J P Denèfle J P Lechaire |
| |
| Institution: | 1. Medical Ethics and Law Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran;3. Department of Biomedical, Surgical and Dental Sciences, Milan State University, Milan, Italy;4. Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol 61663-335, Iran;5. Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;6. Department of Biomedical Sciences, University of Cagliari, Italy;7. Department of Agricultural and Environmental Sciences, Milan State University, Milan, Italy;8. Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;9. Department of Chemistry, Richardson College for the Environmental Science Complex, The University of Winnipeg, Winnipeg, MB, Canada |
| |
| Abstract: | The processes of desmosome formation and keratinization were studied in isolated frog skins cultured in a two-compartment (mucosal and serosal) chamber. Before culture, the skin fragments were trypsinized (stratum corneum together with some parts of stratum granulosum and spinosum were scraped off with forceps) allowing the stratum germinativum to remain on the dermis. When both the mucosal and the serosal culture media contained 1.5 mM calcium and 86 mM sodium concentrations, fully developed desmosomes were differentiated and no keratinization occurred. When the mucosal medium was lowered in two steps to a final calcium concentration of 0.5 mM by dilution with tridistilled sterile water, poorly developed desmosomes were formed, keratinocytes interdigitated and the keratinization was strongly enhanced. The calcium-dependent desmosome formation was affected by the salt gradient established across the skin. These two effects, modulated desmosome formation (calcium) and increased keratinization (sodium), were concomitant with but did not complement one another. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
