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The GATATC-modification enzyme EcoRV is closely related to the GATC-recognizing methyltransferases DpnII and dam from E. coli and phage T4
Authors:R Lauster  A Kriebardis  W Guschlbauer
Institution:1. Max-Planck-Institut für molekulare Genetik, Ihnestrasse 73 D-1000 Berlin 33 Germany;2. Service de Biochimie, Département de Biologie, Bât. 142 Centre d''Etudes Nucléaires de Saclay, F-91191 Gif-sur- Yvette Cédex, France
Abstract:The amino acid sequence of EcoRV DNA methyltransferase which methylates the amino group of the 5'-adenine residue of the target sequence GATATC has been found to be closely related to that of three other adenine methyltransferases, DpnII, dam and damT4, the target sequence of which is GATC. Despite large differences on the DNA level, the four sequences show four blocks of homologies. One of these blocks has the sequence DVYXDPPY and is found with little modification in numerous other DNA methyltransferases. It is speculated that it could be the binding site of the methyl donor, S-adenosylmethionine. On the other hand, the identification of a DNA-binding region is more tenuous. As expected, no analogies with (dimeric) repressors and cro proteins which have the characteristic helix-turn-helix motif have been observed.
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