Activities involved in base excision repair of bacteriophage T4 and lambda DNA in vivo |
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Authors: | Eric H Radany Hong T Nguyen and Kenneth W Minton |
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Institution: | (1) Department of Pathology, F.E. Hebert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd., 20814-4799 Bethesda, MD, USA;(2) Present address: Department of Radiation Therapy, Hospital of the University of Pennsylvania, 3400 Spruce St., 19104 Philadelphia, PA, USA |
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Abstract: | Summary The in vivo excision repair functions of Escherichia coli exonuclease III and 3-methyladenine DNA glycosylase I, and bacteriophage T4 pyrimidine dimer-DNA glycosylase were investigated. Following exposure of bacteriophage T4 or lambda to methyl methanesulfonate or ultraviolet irradiation, survival was determined by plating on E. coli have various genetic backgrounds. Although exonuclease III was shown to participate in base excision repair initiated by 3-methyladenine DNA glcosylase I, it had no detectable role in base excision repair initiated by the T4 pyrimidine dimer-DNA glycosylase. Despite its 3 apurinic/apyrimidinic endonuclease activity in vitro, T4 pyrimidine dimer-DNA glycosylase, even in large quantities, did not complement mutants defective in exonuclease III in the repair of apurinic sites generated by 3-methyladenine DNA glycosylase I in vivo. |
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Keywords: | Excision repair Exonuclease III 3-Methyladenine DNA glycosylase T4 pyrimidine dimer-DNA glycosylase |
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