Demonstration of the presence of G-proteins in hepatic microsomal fraction |
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Authors: | J Codina S Kimura N Kraus-Friedmann |
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Affiliation: | Department of Cell Biology, Baylor College of Medicine, Houston, Texas. |
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Abstract: | The presence of G-proteins in isolated hepatic microsomal vesicles is demonstrated. The G-proteins were identified by their capacity to be ADP-ribosylated by cholera and pertussis toxins. Cholera toxin identified 42 and 45 kDa proteins, corresponding to alpha s-1 and alpha s-2, respectively. Pertussis toxin identified a 40 kDa protein corresponding to alpha i. The microsomal G-proteins are identical to the corresponding G proteins of the plasma membrane, but are present in different proportions; the microsomes have considerably less alpha s proteins than the plasma membrane. |
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