Preparation of highly purified mitochondria ofNeurospora crassa on a Percoll gradient |
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| Authors: | H. Fečíková J. Turňa Z. Baráth |
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| Affiliation: | 1. Institute of Biochemistry, Komensky University, 842 15, Bratislava, Czechoslovakia
2. Department of Biochemistry, Faculty of Sciences, 842 15, Bratislava, Czechoslovakia
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| Abstract: | Mitochondria isolated from Neurospora crassa were purified by centrifugation in a Percoll density gradient. Enzyme activities and cytochrome differential spectra revealed a high purity of the mitochondria. As compared with a crude mitochondrial fraction the purified mitochondria exhibited a high respiratory activity and a fine ADP/O ratio. Electrophoresis of nucleic acids demonstrated the absence of cytoplasmic rRNA. |
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